Coding

Part:BBa_K1903004:Design

Designed by: Sofía Vieto Fonseca   Group: iGEM16_TEC-Costa_Rica   (2016-10-14)


GFP-TEVlinker-Quencher


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

This is a FRET system to quenched the GFP E0040 using a Dark quencher REACH1BBa_K1319001, characterized by Team Aachen 2014, as an acceptor of the FRET system. The GFP and the Dark Quencher are linked by a TEV cleavage site K1159318.

Source

We use the part registry following accessions: GFP E0040 , TEV cleavage site K1159318 and REACh1 K1319001.

References